BD Pharmingen™ Leukocyte Blocking Buffer reduces nonspecific binding of cyanine-like dye conjugates to monocytes and other leukocytes

Abstract Nonspecific binding of monocytes to certain cyanine-like dye conjugated antibodies that are commonly used in flow cytometry for cell surface staining is well known. Although lesser extent than monocytes, other leukocyte subsets can also exhibit nonspecific conjugates. This poses a challenge multicolor cytometric analysis utilizing these conjugates as dye-mediated mask the real expression. Here we demonstrate BD Pharmingen™ Leukocyte Blocking Buffer, blocking buffer solution, eliminates and populations. In this study show human whole blood stained with PE-Cy5, PE-Cy7, APC-Cy7, APC-H7, PE-CF594 tandem-dye presence new exhibits appropriate profile subsets. We observed myeloid lymphoid mouse tissues was diminished. while reducing binding, did not impact desirable antibody-mediated specific staining. Our data viability impacted. For Research Use Only. Not use diagnostic or therapeutic procedures. Pharmingen trademarks Becton, Dickinson Company its affiliates. © 2023 BD. All rights reserved. NPM-0263 (v1.0) 1222 CF trademark Biotium, Inc. Cy Global Life Sciences Solutions Germany GmbH an affiliate doing business Cytiva.